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ribT [2017-12-18 09:49:00]
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ribT [2017-12-18 09:49:00]

reductase
locus
BSU23240
pI
5.75
mw
14.43 kDa
protein length
124 aa Sequence Blast
gene length
372 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
ribD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    2,427,405 → 2,427,779

    Expression and Regulation

    Operons

    genes
    [gene|3DFCF7C5FE15C9A3E9E7E4B0DA8E926B85DBD180|ribD]-[gene|4E18BD4B084094EE4FBC8FE2AC95B14581D20C0F|ribE]-[gene|F07B7062C850106A95C15978E36DA500F8B089D6|ribA]-[gene|2A5DCBFAB51996F0DDEC964D5D178274AF86CE69|ribH]-[gene|8146A052E4E7844F781E7169C264EC927DEB7387|ribT]
    description
    [Pubmed|8159171]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8159171], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [regulon|FMN-box|FMN-box]: transcription termination, via [SW|FMN-box] in the presence of FMN or FMNH2, this is counter-acted upon binding of [protein|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|RibR], in [regulon|FMN-box|FMN-box]

    • regulation

  • expressed in the absence of FMN ([SW|FMN-box]) [Pubmed|15808508]
  • binding of FMN to the [SW|FMN-box] RNA is facilitated by [protein|43CFA3477B4EEB4CE0B2DF63ED2B5EDB171E79C7|NusG]-mediated transcription pausing [pubmed|32817529]
  • the [SW|FMN-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
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    Biological materials

    Mutant

  • BKE23240 (Δ[gene|8146A052E4E7844F781E7169C264EC927DEB7387|ribT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAACCCCTCTATATC, downstream forward: _UP4_TAAGCAGAGGCTGTGATCAG
  • BKK23240 (Δ[gene|8146A052E4E7844F781E7169C264EC927DEB7387|ribT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAACCCCTCTATATC, downstream forward: _UP4_TAAGCAGAGGCTGTGATCAG

    • References

  • 12456892,15808508,23270261,8159171,7934830,24442413,25349719,26494285